Hotspot

trnK-UUU-rps16

LSC · 4207-5051 · 845 bp

Back to Ammannia

Pi 0.0401
MarkerSeek score 75.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
845
variable_sites
50
pi
0.0401
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT ACAGAATTTCATTTTAATCAAACG 50.0 25.0 58.2 53.2 910-934 bp 1.0 46.1
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT ACAGAATTTCATTTTAATCAAACG 50.0 25.0 58.2 53.2 909-933 bp 1.0 46.1
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT CAGAATTTCATTTTAATCAAACGA 50.0 25.0 58.2 52.9 909-933 bp 1.0 46.1
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT CAGAATTTCATTTTAATCAAACGA 50.0 25.0 58.2 52.9 908-932 bp 1.0 46.1
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA ACAGAATTTCATTTTAATCAAACG 50.0 25.0 58.3 53.2 919-943 bp 1.0 46.0