Hotspot

trnH-GUG-psbA

LSC · 77-334 · 258 bp

Back to Ammannia

Pi 0.0413

MarkerSeek score 69.90

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 258
variable_sites: 15
pi: 0.0413
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CTGTCGAAGCTCCATCTACA	55.0	50.0	58.1	56.8	308-324 bp	1.0	76.9
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CTGTCGAAGCTCCATCTACA	50.0	50.0	57.9	56.8	322-338 bp	1.0	76.7
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CTGTCGAAGCTCCATCTACA	50.0	50.0	57.9	56.8	321-337 bp	1.0	76.7
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CTGTCGAAGCTCCATCTACA	50.0	50.0	57.9	56.8	323-339 bp	1.0	76.7
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CTGTCGAAGCTCCATCTACAA	55.0	47.6	58.1	57.5	308-324 bp	1.0	74.7