Hotspot

ycf1-chlN

Genome · 132189-133117 · 929 bp

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Pi 0.00596

MarkerSeek score 58.20

Primer available yes

Label ycf1-chlN

Feature Metadata

label_name: ycf1-chlN
feature_type: igs
region: Genome
length_bp: 929
variable_sites: 13
pi: 0.00596
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-chlN_p1	CAAGGTCGTCAAGAACCCAT	GAAGACTTGGATCGGACCAC	50.0	55.0	57.8	58.0	1105-1147 bp	1.0	80.7
ycf1-chlN_p2	CAAGGTCGTCAAGAACCCAT	AATTGGTTACCCGACCCCTA	50.0	50.0	57.8	58.0	1143-1185 bp	1.0	80.4
ycf1-chlN_p3	CAAGGTCGTCAAGAACCCAT	ACTTGGATCGGACCACCTTA	50.0	50.0	57.8	58.1	1101-1143 bp	1.0	80.3
ycf1-chlN_p4	CAAGGTCGTCAAGAACCCAT	TTGGATCGGACCACCTTAGT	50.0	50.0	57.8	58.1	1099-1141 bp	1.0	80.3
ycf1-chlN_p5	CAAGGTCGTCAAGAACCCAT	AGAAGACTTGGATCGGACCA	50.0	50.0	57.8	58.1	1106-1148 bp	1.0	80.3