Hotspot

rrn4.5-rrn5

Genome · 109322-109661 · 340 bp

Back to Amentotaxus

Pi 0.0098

MarkerSeek score 42.70

Primer available yes

Label rrn4.5-rrn5

Feature Metadata

label_name: rrn4.5-rrn5
feature_type: igs
region: Genome
length_bp: 340
variable_sites: 8
pi: 0.0098
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn5_p1	CAGCTGAGGCATCCTAACAG	GCATCGAGCTATTTTTCCGC	55.0	50.0	58.0	58.0	490-493 bp	1.0	80.9
rrn4.5-rrn5_p2	GCTGAGGCATCCTAACAGAC	GCATCGAGCTATTTTTCCGC	55.0	50.0	58.1	58.0	488-491 bp	1.0	80.9
rrn4.5-rrn5_p3	CAGCTGAGGCATCCTAACAG	AGTATCGTCACCGCAGTAGA	55.0	50.0	58.0	58.0	456-459 bp	1.0	80.9
rrn4.5-rrn5_p4	CAGCTGAGGCATCCTAACAG	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.0	58.0	454-457 bp	1.0	80.9
rrn4.5-rrn5_p5	GCTGAGGCATCCTAACAGAC	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.1	58.0	452-455 bp	1.0	80.9