Hotspot

rps12-trnV-GAC

Genome · 99874-100861 · 988 bp

Back to Amentotaxus

Pi 0.00405
MarkerSeek score 56.90
Primer available yes
Label rps12-trnV-GAC

Feature Metadata

label_name
rps12-trnV-GAC
feature_type
igs
region
Genome
length_bp
988
variable_sites
10
pi
0.00405
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GAC_p1 TGGCAACTTTACGTAAGGCA GACTTTCACCACGTCAAGGT 45.0 50.0 57.7 58.1 1071-1081 bp 1.0 79.1
rps12-trnV-GAC_p2 TGGCAACTTTACGTAAGGCA TGACTTTCACCACGTCAAGG 45.0 50.0 57.7 58.1 1072-1082 bp 1.0 79.1
rps12-trnV-GAC_p3 TGGCAACTTTACGTAAGGCA CGATGACTTTCACCACGTCA 45.0 50.0 57.7 58.2 1075-1085 bp 1.0 77.7
rps12-trnV-GAC_p4 ACGTAAGGCAGAGTTTGGTT GACTTTCACCACGTCAAGGT 45.0 50.0 57.4 58.1 1061-1071 bp 1.0 76.1
rps12-trnV-GAC_p5 ACGTAAGGCAGAGTTTGGTT TGACTTTCACCACGTCAAGG 45.0 50.0 57.4 58.1 1062-1072 bp 1.0 76.1