Hotspot

ycf2-trnL-CAA

Genome · 94888-95467 · 580 bp

Back to Amentotaxus

Pi 0.00466
MarkerSeek score 56.90
Primer available yes
Label ycf2-trnL-CAA

Feature Metadata

label_name
ycf2-trnL-CAA
feature_type
igs
region
Genome
length_bp
580
variable_sites
7
pi
0.00466
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnL-CAA_p1 TCGGAATGACATTCACTGCT AAATGGTAGACACGCGAGAC 45.0 50.0 57.2 58.0 662-707 bp 1.0 78.6
ycf2-trnL-CAA_p2 TCGGAATGACATTCACTGCT TGAAATGGTAGACACGCGAG 45.0 50.0 57.2 58.0 664-709 bp 1.0 78.6
ycf2-trnL-CAA_p3 ATGGCTTTCTCGGAATGACA AAATGGTAGACACGCGAGAC 45.0 50.0 57.2 58.0 671-716 bp 1.0 78.4
ycf2-trnL-CAA_p4 TGGCTTTCTCGGAATGACAT AAATGGTAGACACGCGAGAC 45.0 50.0 57.2 58.0 670-715 bp 1.0 78.4
ycf2-trnL-CAA_p5 TGGCTTTCTCGGAATGACAT TGAAATGGTAGACACGCGAG 45.0 50.0 57.2 58.0 672-717 bp 1.0 78.4