Hotspot

psbJ-petA

Genome · 68202-69210 · 1009 bp

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Pi 0.00479

MarkerSeek score 63.30

Primer available yes

Label psbJ-petA

Feature Metadata

label_name: psbJ-petA
feature_type: igs
region: Genome
length_bp: 1009
variable_sites: 12
pi: 0.00479
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-petA_p1	TCCGGATTAGGATCATCCCT	TAGCATCTGTCGTTTTGGCA	50.0	45.0	57.0	57.8	1112-1123 bp	1.0	72.1
psbJ-petA_p2	TCCGGATTAGGATCATCCCT	TCTGTCGTTTTGGCACAGAT	50.0	45.0	57.0	57.7	1107-1118 bp	1.0	71.5
psbJ-petA_p3	TCCGGATTAGGATCATCCCT	CATCTGTCGTTTTGGCACAG	50.0	50.0	57.0	57.7	1109-1120 bp	1.0	71.0
psbJ-petA_p4	TTCCGGATTAGGATCATCCCT	TAGCATCTGTCGTTTTGGCA	47.6	45.0	57.7	57.8	1113-1124 bp	1.0	70.1
psbJ-petA_p5	TTCCGGATTAGGATCATCCCT	TCTGTCGTTTTGGCACAGAT	47.6	45.0	57.7	57.7	1108-1119 bp	1.0	69.5