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clpP-rps12

Genome · 61900-62507 · 608 bp

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Pi 0.0198

MarkerSeek score 73.70

Primer available yes

Label clpP-rps12

Feature Metadata

label_name: clpP-rps12
feature_type: igs
region: Genome
length_bp: 608
variable_sites: 33
pi: 0.0198
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-rps12_p1	AATTTGGCCTTGTCGACCAA	TTCTGTATTGGCTGTCTCGC	45.0	50.0	58.3	58.0	768-774 bp	1.0	80.9
clpP-rps12_p2	TTTGGCCTTGTCGACCAAAT	TTCTGTATTGGCTGTCTCGC	45.0	50.0	58.3	58.0	766-772 bp	1.0	80.9
clpP-rps12_p3	AATTTGGCCTTGTCGACCAA	CCTTTACGCTGAGGACATCC	45.0	55.0	58.3	58.1	813-819 bp	1.0	80.4
clpP-rps12_p4	TTTGGCCTTGTCGACCAAAT	CCTTTACGCTGAGGACATCC	45.0	55.0	58.3	58.1	811-817 bp	1.0	80.4
clpP-rps12_p5	AGAATTTGGCCTTGTCGACC	TTCTGTATTGGCTGTCTCGC	50.0	50.0	58.5	58.0	770-776 bp	1.0	79.6