Hotspot

ndhC-trnV-UAC

Genome · 50032-52002 · 1971 bp

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Pi 0.00268

MarkerSeek score 60.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: Genome
length_bp: 1971
variable_sites: 15
pi: 0.00268
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TTACTTATGGGGGCCAAAGC	TGGGTTCTTGGAACAGTTCG	50.0	50.0	57.8	58.0	2164-2169 bp	1.0	79.4
ndhC-trnV-UAC_p2	TTACTTATGGGGGCCAAAGC	TGCATGTTGGGTTCTTGGAA	50.0	45.0	57.8	57.9	2171-2176 bp	1.0	79.1
ndhC-trnV-UAC_p3	GGGGCCAAAGCTATGGAAAT	TGGGTTCTTGGAACAGTTCG	50.0	50.0	58.2	58.0	2155-2160 bp	1.0	79.0
ndhC-trnV-UAC_p4	GGGGCCAAAGCTATGGAAAT	TGCATGTTGGGTTCTTGGAA	50.0	45.0	58.2	57.9	2162-2167 bp	1.0	78.6
ndhC-trnV-UAC_p5	CTCCGGCCCTTTACTTATGG	TGGGTTCTTGGAACAGTTCG	55.0	50.0	57.7	58.0	2174-2179 bp	1.0	78.6