Hotspot

trnL-UAA-trnF-GAA

Genome · 47428-47831 · 404 bp

Back to Amentotaxus

Pi 0.00693
MarkerSeek score 46.90
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
Genome
length_bp
404
variable_sites
7
pi
0.00693
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 TGCAGTAGGAGGAAAATCCG TGGCACGAGGATTTTCAGTC 50.0 50.0 57.3 58.2 514-516 bp 1.0 74.2
trnL-UAA-trnF-GAA_p2 GCAGTAGGAGGAAAATCCGT TGGCACGAGGATTTTCAGTC 50.0 50.0 57.3 58.2 513-515 bp 1.0 74.2
trnL-UAA-trnF-GAA_p3 TGCAGTAGGAGGAAAATCCG AATAATGGTTCCGCATGCCA 50.0 45.0 57.3 58.2 553-555 bp 1.0 73.8
trnL-UAA-trnF-GAA_p4 GCAGTAGGAGGAAAATCCGT AATAATGGTTCCGCATGCCA 50.0 45.0 57.3 58.2 552-554 bp 1.0 73.8
trnL-UAA-trnF-GAA_p5 TGCAGTAGGAGGAAAATCCG CCAGATTTGAACTGGTGGCA 50.0 50.0 57.3 58.4 529-531 bp 1.0 72.6