Hotspot

atpH-atpI

Genome · 14154-14826 · 673 bp

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Pi 0.00396

MarkerSeek score 56.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: Genome
length_bp: 673
variable_sites: 8
pi: 0.00396
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCAATAGAAGCGAGGCCTA	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	766-768 bp	1.0	79.0
atpH-atpI_p2	CCTACGGATAATCCAGCAGC	CGAATCCATGGAGGGTCATC	55.0	55.0	57.9	57.8	750-752 bp	1.0	79.0
atpH-atpI_p3	GCCTACGGATAATCCAGCAG	CGAATCCATGGAGGGTCATC	55.0	55.0	57.9	57.8	751-753 bp	1.0	79.0
atpH-atpI_p4	AACGGAAGCAGCAGAAATCA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.1	57.8	727-729 bp	1.0	78.9
atpH-atpI_p5	ACGGAAGCAGCAGAAATCAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.1	57.8	726-728 bp	1.0	78.9