Hotspot

psbI-trnS-GCU

Genome · 8753-8810 · 58 bp

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Pi 0.0269

MarkerSeek score 59.70

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: Genome
length_bp: 58
variable_sites: 4
pi: 0.0269
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GGAATCCTGGGCGTAAAGAA	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.6	58.2	102-104 bp	1.0	79.6
psbI-trnS-GCU_p2	GGAATCCTGGGCGTAAAGAA	TTCGAATCCCTCTCTCTCCG	50.0	55.0	57.6	58.3	99-101 bp	1.0	79.0
psbI-trnS-GCU_p3	GGAATCCTGGGCGTAAAGAA	GACTAAAGCGGTGGATTGCT	50.0	50.0	57.6	58.3	150-152 bp	1.0	77.8
psbI-trnS-GCU_p4	GGAATCCTGGGCGTAAAGAA	GATGGCTGAGCGGACTAAAG	50.0	55.0	57.6	58.4	162-164 bp	1.0	76.3
psbI-trnS-GCU_p5	TTATCGAACGATCCAGGACG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.2	58.2	121-123 bp	1.0	76.1