Hotspot

rpl16

LSC · 84881-86270 · 1390 bp

Back to Amelanchier

Pi 0.00535

MarkerSeek score 32.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1390
variable_sites: 17
pi: 0.00535
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACCCCTTTCGTTTGTTTCTACA	TGGCAAACTCTTTCATTCTT	40.9	35.0	58.1	53.3	1510-1528 bp	1.0	41.4
rpl16_p2	ACCCCTTTCGTTTGTTTCTACA	TGGCAAACTCTTTCATTCTTT	40.9	33.3	58.1	54.2	1510-1528 bp	1.0	41.4
rpl16_p3	AACCCCTTTCGTTTGTTTCT	TGGCAAACTCTTTCATTCTT	40.0	35.0	55.7	53.3	1511-1529 bp	1.0	41.4
rpl16_p4	ACCCCTTTCGTTTGTTTCTACA	ATGGCAAACTCTTTCATTCT	40.9	35.0	58.1	53.0	1511-1529 bp	1.0	41.4
rpl16_p5	ACCCCTTTCGTTTGTTTCTACA	TGGCAAACTCTTTCATTCTTTT	40.9	31.8	58.1	55.0	1510-1528 bp	1.0	41.4