Hotspot

rpl32-trnL-UAG

SSC · 117732-119151 · 1420 bp

Back to Amelanchier

Pi 0.00637
MarkerSeek score 45.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1420
variable_sites
25
pi
0.00637
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCATTGAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 1513-1629 bp 1.0 82.2
rpl32-trnL-UAG_p2 GGGCAGCATTGAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 1514-1630 bp 1.0 82.1
rpl32-trnL-UAG_p3 TAGGGCAGCATTGAAAGCTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 1515-1631 bp 1.0 81.8
rpl32-trnL-UAG_p4 TAGGGCAGCATTGAAAGCTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 1516-1632 bp 1.0 81.8
rpl32-trnL-UAG_p5 GGGCAGCATTGAAAGCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.8 57.9 1523-1639 bp 1.0 81.2