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rpl33-rps18

LSC · 71249-71515 · 267 bp

Back to Amelanchier

Pi 0.0354

MarkerSeek score 72.40

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 267
variable_sites: 21
pi: 0.0354
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGACACAATACGCCTAGTCG	TCAATTCGATCCCCCGATTG	55.0	50.0	58.5	57.7	381-465 bp	1.0	81.6
rpl33-rps18_p2	GACACAATACGCCTAGTCGA	TCAATTCGATCCCCCGATTG	50.0	50.0	57.2	57.7	380-464 bp	1.0	79.2
rpl33-rps18_p3	TCGACACAATACGCCTAGTC	TCAATTCGATCCCCCGATTG	50.0	50.0	57.2	57.7	382-466 bp	1.0	79.2
rpl33-rps18_p4	CGACACAATACGCCTAGTCG	ATTCGATCCCCCGATTGGAT	55.0	50.0	58.5	58.6	378-462 bp	1.0	78.6
rpl33-rps18_p5	AGAATCGACACAATACGCCT	TCAATTCGATCCCCCGATTG	45.0	50.0	57.0	57.7	386-470 bp	1.0	78.2