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psbE-petL

LSC · 68010-69241 · 1232 bp

Back to Amelanchier

Pi 0.00194

MarkerSeek score 51.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1232
variable_sites: 8
pi: 0.00194
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCG	50.0	55.0	57.8	58.2	1343-1391 bp	1.0	78.7
psbE-petL_p2	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCG	45.0	55.0	58.2	58.2	1429-1477 bp	1.0	78.5
psbE-petL_p3	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCG	50.0	55.0	58.2	58.2	1434-1482 bp	1.0	78.4
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCG	55.0	55.0	58.2	58.2	1338-1386 bp	1.0	78.4
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	TCGTATCTTGCTCAGACCGA	50.0	50.0	57.8	58.3	1342-1390 bp	1.0	78.0