Hotspot

psbZ-trnG-UCC

LSC · 38770-39279 · 510 bp

Back to Amelanchier

Pi 0.0152

MarkerSeek score 48.30

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 510
variable_sites: 20
pi: 0.0152
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	597-700 bp	1.0	83.1
psbZ-trnG-UCC_p2	GGGTTGGTCGGGTAACAAAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	583-686 bp	1.0	82.9
psbZ-trnG-UCC_p3	TTCCGGCACATCATTATGGA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.0	57.8	553-656 bp	1.0	77.3
psbZ-trnG-UCC_p4	AGGGTTGGTCGGGTAACAAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	59.2	57.8	584-687 bp	1.0	76.1
psbZ-trnG-UCC_p5	ATTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.2	57.8	598-701 bp	1.0	75.7