Hotspot

rpoA

LSC · 77976-78983 · 1008 bp

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Pi 0.0129

MarkerSeek score 66.20

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1008
variable_sites: 13
pi: 0.0129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TCAAAACCTATTTTCGGCCCT	AGCAAGAGAAACAAGAGAGCA	42.9	42.9	57.8	57.5	1133-1134 bp	1.0	59.8
rpoA_p2	ACCTATTTTCGGCCCTTTTCT	AGCAAGAGAAACAAGAGAGCA	42.9	42.9	57.5	57.5	1128-1129 bp	1.0	57.5
rpoA_p3	TCAAAACCTATTTTCGGCCCT	GCAAGAGAAACAAGAGAGCA	42.9	45.0	57.8	56.0	1132-1133 bp	1.0	55.5
rpoA_p4	TCAAAACCTATTTTCGGCCC	AGCAAGAGAAACAAGAGAGCA	45.0	42.9	56.3	57.5	1133-1134 bp	1.0	55.5
rpoA_p5	CAAAACCTATTTTCGGCCCT	AGCAAGAGAAACAAGAGAGCA	45.0	42.9	56.3	57.5	1132-1133 bp	1.0	55.4