Hotspot

rbcL

LSC · 55102-56559 · 1458 bp

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Pi 0.00206

MarkerSeek score 46.10

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1458
variable_sites: 3
pi: 0.00206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	GTACCATCCAACCGAATCCA	GACCAAGACTGCTCGTTGAT	50.0	50.0	57.3	57.9	2789-2790 bp	1.0	74.8
rbcL_p2	GTACCATCCAACCGAATCCA	TGTCTCGATCCAGGGAAACT	50.0	50.0	57.3	58.1	2368-2372 bp	1.0	74.5
rbcL_p3	GTACCATCCAACCGAATCCA	TGGGCCGAGTTTCATTGAAA	50.0	45.0	57.3	58.0	2161 bp	0.5	54.3
rbcL_p4	GTACCATCCAACCGAATCCA	TTGGGCCGAGTTTCATTGAA	50.0	45.0	57.3	58.0	2162 bp	0.5	54.3
rbcL_p5	ACCATCCAACCGAATCCAAT	TGGGCCGAGTTTCATTGAAA	45.0	45.0	57.2	58.0	2159 bp	0.5	53.3