Hotspot

ndhC-trnV-UAC

LSC · 50332-51317 · 986 bp

Back to Ambrosia

Pi 0.0101

MarkerSeek score 61.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 986
variable_sites: 10
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACTCGGAGCTAAAAATCCCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1121 bp	1.0	76.6
ndhC-trnV-UAC_p2	CTCGGAGCTAAAAATCCCGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.4	57.8	1120 bp	1.0	74.4
ndhC-trnV-UAC_p3	ACTCGGAGCTAAAAATCCCG	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.7	57.5	1120 bp	1.0	73.9
ndhC-trnV-UAC_p4	ACTCGGAGCTAAAAATCCCG	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.7	58.7	1109 bp	1.0	72.8
ndhC-trnV-UAC_p5	CCCTTTGCTACTCGGAGCTA	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.9	57.8	1130 bp	1.0	72.0