Hotspot

trnG-UCC-trnT-GGU

LSC · 31391-31531 · 141 bp

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Pi 0.0496
MarkerSeek score 69.60
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
141
variable_sites
7
pi
0.0496
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 GGGGGTTAATAGAACGAATCACA ACGCGTTACCATGGGATTAC 43.5 50.0 58.2 58.1 226 bp 1.0 56.2
trnG-UCC-trnT-GGU_p2 GGGGGTTAATAGAACGAATCACA CACGCGTTACCATGGGATTA 43.5 50.0 58.2 58.1 227 bp 1.0 56.2
trnG-UCC-trnT-GGU_p3 GGGGGTTAATAGAACGAATCACA CGCGTTACCATGGGATTACT 43.5 50.0 58.2 57.8 225 bp 1.0 54.8
trnG-UCC-trnT-GGU_p4 GGGGGTTAATAGAACGAATCAC ACGCGTTACCATGGGATTAC 45.5 50.0 56.6 58.1 226 bp 1.0 54.7
trnG-UCC-trnT-GGU_p5 GGGGGTTAATAGAACGAATCAC CACGCGTTACCATGGGATTA 45.5 50.0 56.6 58.1 227 bp 1.0 54.6