Hotspot

trnK-UUU-rps16

LSC · 4540-5401 · 862 bp

Back to Ambrosia

Pi 0.0128
MarkerSeek score 55.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
862
variable_sites
11
pi
0.0128
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC GGAACTTTGCCCTAATCAAACG 55.0 45.5 57.9 58.2 991 bp 1.0 62.9
trnK-UUU-rps16_p2 CGATGGTATGGACGAATCCC GGAACTTTGCCCTAATCAAACG 55.0 45.5 57.9 58.2 990 bp 1.0 62.9
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GGAACTTTGCCCTAATCAAACG 50.0 45.5 58.2 58.2 930 bp 1.0 62.3
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GGAACTTTGCCCTAATCAAACG 50.0 45.5 58.2 58.2 929 bp 1.0 62.3
trnK-UUU-rps16_p5 TCAGTCGTGGTCTTCCAAAC GGAACTTTGCCCTAATCAAACG 50.0 45.5 57.8 58.2 1015 bp 1.0 61.7