Hotspot

rps19-trnH-GUG

LSC · 152-181 · 30 bp

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Pi 0.125
MarkerSeek score 70.00
Primer available yes
Label rps19-trnH-GUG

Feature Metadata

label_name
rps19-trnH-GUG
feature_type
igs
region
LSC
length_bp
30
variable_sites
1
pi
0.125
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.27
markerseek_score
70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-trnH-GUG_p1 GAGGAAGCTGTGACACGTTC CGGATGTAGCCAAGTGGATC 55.0 55.0 58.9 58.1 201 bp 0.5 52.1
rps19-trnH-GUG_p2 GAGGAAGCTGTGACACGTTC TGGATCAAGGCAGTGGATTG 55.0 50.0 58.9 57.9 187 bp 0.5 52.1
rps19-trnH-GUG_p3 GAGGAAGCTGTGACACGTTC GGATCAAGGCAGTGGATTGT 55.0 50.0 58.9 57.9 186 bp 0.5 52.0
rps19-trnH-GUG_p4 GAGGAAGCTGTGACACGTTC GTGGATCAAGGCAGTGGATT 55.0 50.0 58.9 57.9 188 bp 0.5 52.0
rps19-trnH-GUG_p5 GGAAGCTGTGACACGTTCA CGGATGTAGCCAAGTGGATC 52.6 55.0 58.0 58.1 199 bp 0.5 50.9