Hotspot

rpl32-trnL-UAG

SSC · 111689-112867 · 1179 bp

Back to Amaranthus

Pi 0.0211
MarkerSeek score 65.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1179
variable_sites
107
pi
0.0211
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCAGCGTTAAAAGCCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 1111-1305 bp 1.0 89.6
rpl32-trnL-UAG_p2 GGCAGCGTTAAAAGCCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 1112-1306 bp 1.0 89.6
rpl32-trnL-UAG_p3 GGCAGCGTTAAAAGCCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 1121-1315 bp 1.0 88.6
rpl32-trnL-UAG_p4 GGCAGCGTTAAAAGCCTTTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.9 57.8 1120-1314 bp 1.0 88.1
rpl32-trnL-UAG_p5 GGCAGCGTTAAAAGCCTTTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.9 57.8 1119-1313 bp 1.0 88.1