Hotspot

rpl33-rps18

LSC · 67269-67553 · 285 bp

Back to Amaranthus

Pi 0.0123
MarkerSeek score 58.30
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
285
variable_sites
13
pi
0.0123
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 CTAGTCGGTTGGAATTGCGA CAAACGCCTACGAAAAGCTC 50.0 50.0 58.0 57.8 398-432 bp 1.0 84.6
rpl33-rps18_p2 CTAGTCGGTTGGAATTGCGA TATTCGATCCCCCGATTGGA 50.0 50.0 58.0 58.0 425-459 bp 0.95 84.1
rpl33-rps18_p3 GACACAATACGCCTAGTCGG TATTCGATCCCCCGATTGGA 55.0 50.0 58.2 58.0 437-471 bp 0.95 82.8
rpl33-rps18_p4 ACAATACGCCTAGTCGGTTG TATTCGATCCCCCGATTGGA 50.0 50.0 57.7 58.0 434-468 bp 0.95 81.8
rpl33-rps18_p5 CACAATACGCCTAGTCGGTT TATTCGATCCCCCGATTGGA 50.0 50.0 57.7 58.0 435-469 bp 0.95 81.8