Hotspot

psbE-petL

LSC · 64458-65328 · 871 bp

Back to Amaranthus

Pi 0.0104

MarkerSeek score 58.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 871
variable_sites: 48
pi: 0.0104
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCGGAGGTTATAGTTAAAGCC	50.0	45.5	57.9	57.9	1032-1061 bp	1.0	67.2
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GGAGGTTATAGTTAAAGCCGCT	50.0	45.5	57.9	57.9	1029-1058 bp	1.0	67.2
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GGAGGTTATAGTTAAAGCCGCT	50.0	45.5	57.8	57.9	952-981 bp	1.0	66.7
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AGCGGAGGTTATAGTTAAAGCC	50.0	45.5	57.8	57.9	955-984 bp	1.0	66.6
psbE-petL_p5	ACGAATAACCAACCCGCAAT	GGAGGTTATAGTTAAAGCCGCT	45.0	45.5	58.2	57.9	1038-1067 bp	1.0	66.2