Hotspot

accD-psaI

LSC · 59002-59522 · 521 bp

Back to Amaranthus

Pi 0.0156

MarkerSeek score 65.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 521
variable_sites: 30
pi: 0.0156
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTGATCCAATCGTACCACGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.0	58.0	655-696 bp	1.0	79.1
accD-psaI_p2	TTTGATCCAATCGTACCACGT	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.7	58.0	656-697 bp	1.0	76.9
accD-psaI_p3	TTGATCCAATCGTACCACGT	GCCGGAAATACTAGGCCTAC	45.0	55.0	57.0	57.5	644-685 bp	1.0	75.8
accD-psaI_p4	TTGATCCAATCGTACCACGT	AAAGACGCCATTGCAATTGC	45.0	45.0	57.0	58.6	662-703 bp	1.0	74.7
accD-psaI_p5	TTTGATCCAATCGTACCACGT	GCCGGAAATACTAGGCCTAC	42.9	55.0	57.7	57.5	645-686 bp	1.0	73.5