Hotspot

trnT-trnL-UAA

LSC · 46783-47435 · 653 bp

Back to Amaranthus

Pi 0.0107

MarkerSeek score 57.80

Primer available yes

Label trnT-trnL-UAA

Feature Metadata

label_name: trnT-trnL-UAA
feature_type: igs
region: LSC
length_bp: 653
variable_sites: 36
pi: 0.0107
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	AGCCAATTAAGTCCGTAGCG	55.0	50.0	57.9	58.1	709-736 bp	1.0	85.3
trnT-trnL-UAA_p2	AAAGCCAGCTATCGGAATCG	AGCCAATTAAGTCCGTAGCG	50.0	50.0	58.1	58.1	754-781 bp	1.0	84.9
trnT-trnL-UAA_p3	GGAATCGAACCGATGACCAT	AGCCAATTAAGTCCGTAGCG	50.0	50.0	57.8	58.1	741-768 bp	1.0	84.1
trnT-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	697-724 bp	1.0	83.9
trnT-trnL-UAA_p5	AAAGCCAGCTATCGGAATCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.1	58.2	742-769 bp	1.0	83.5