Hotspot

rps4-trnT

LSC · 46245-46709 · 465 bp

Back to Amaranthus

Pi 0.0126

MarkerSeek score 61.30

Primer available yes

Label rps4-trnT

Feature Metadata

label_name: rps4-trnT
feature_type: igs
region: LSC
length_bp: 465
variable_sites: 25
pi: 0.0126
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT_p1	AGTCCAGGTAAAGCCCCTAG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.1	57.9	584-604 bp	1.0	83.2
rps4-trnT_p2	GTCCAGGTAAAGCCCCTAGA	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.1	57.9	583-603 bp	1.0	83.2
rps4-trnT_p3	TCCAGGTAAAGCCCCTAGAC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.1	57.9	582-602 bp	1.0	83.2
rps4-trnT_p4	AGTCCAGGTAAAGCCCCTAG	GGTTCGATTCCGATAGCTGG	55.0	55.0	58.1	58.2	543-563 bp	1.0	83.0
rps4-trnT_p5	TCCAGGTAAAGCCCCTAGAC	GGTTCGATTCCGATAGCTGG	55.0	55.0	58.1	58.2	541-561 bp	1.0	83.0