Hotspot

psaA-ycf3

LSC · 42004-42773 · 770 bp

Back to Amaranthus

Pi 0.0099

MarkerSeek score 65.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 770
variable_sites: 29
pi: 0.0099
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGGCCCATTCTTCAAACGAA	ATCAAGCCGCTGAGTATTGG	45.0	50.0	58.0	58.0	933-974 bp	1.0	84.9
psaA-ycf3_p2	TGGCCCATTCTTCAAACGAA	ATTGGTTGAAGATCACGGGG	45.0	50.0	58.0	57.9	867-908 bp	1.0	84.6
psaA-ycf3_p3	GGTCTGGCCCATTCTTCAAA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	937-978 bp	1.0	84.4
psaA-ycf3_p4	TGGCCCATTCTTCAAACGAA	CGATCAAGCCGCTGAGTATT	45.0	50.0	58.0	58.1	935-976 bp	1.0	84.1
psaA-ycf3_p5	GGTCTGGCCCATTCTTCAAA	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.1	57.9	871-912 bp	1.0	84.1