Hotspot

trnE-UUC-trnT

LSC · 31001-31494 · 494 bp

Back to Amaranthus

Pi 0.0146

MarkerSeek score 63.40

Primer available yes

Label trnE-UUC-trnT

Feature Metadata

label_name: trnE-UUC-trnT
feature_type: igs
region: LSC
length_bp: 494
variable_sites: 29
pi: 0.0146
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT_p1	GAATCCCCGTTGTCTCCTTG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	601-638 bp	1.0	82.5
trnE-UUC-trnT_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	572-609 bp	1.0	82.5
trnE-UUC-trnT_p3	GAATCCCCGTTGTCTCCTTG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.3	58.1	580-617 bp	1.0	82.2
trnE-UUC-trnT_p4	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	551-588 bp	1.0	82.2
trnE-UUC-trnT_p5	GAATCCCCGTTGTCTCCTTG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.3	58.1	577-614 bp	1.0	82.0