Hotspot

atpH-atpI

LSC · 13467-14214 · 748 bp

Back to Amaranthus

Pi 0.00758

MarkerSeek score 60.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 748
variable_sites: 26
pi: 0.00758
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTGCAACTTTAGCTGCAGC	45.0	45.0	58.0	57.9	822-837 bp	1.0	83.3
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	793-808 bp	1.0	83.2
atpH-atpI_p3	AGCGGCAGAAATCAATGGAT	TTTGCAACTTTAGCTGCAGC	45.0	45.0	57.9	57.9	814-829 bp	1.0	83.1
atpH-atpI_p4	AGCGGCAGAAATCAATGGAT	CGAATCCATGGAGGGTCATC	45.0	55.0	57.9	57.8	785-800 bp	1.0	83.0
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	TTTGCAACTTTAGCTGCAGC	50.0	45.0	57.9	57.9	878-893 bp	1.0	82.4