Hotspot

rpoA

LSC · 76102-77169 · 1068 bp

Pi 0.00633

MarkerSeek score 39.90

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1068
variable_sites: 22
pi: 0.00633
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	GGATCCATTTGCGACTCCTT	GTAGTTGATCAGGGCATGCA	50.0	50.0	58.0	58.0	1813-1828 bp	1.0	80.7
rpoA_p2	GGATCCATTTGCGACTCCTT	TACGAGCCATCCGTAGAAGT	50.0	50.0	58.0	58.0	1743-1758 bp	1.0	80.6
rpoA_p3	GGATCCATTTGCGACTCCTT	GCGATGTAACACCTATGCCA	50.0	50.0	58.0	58.1	1701-1716 bp	1.0	80.6
rpoA_p4	GGATCCATTTGCGACTCCTT	TAGTTCGCGTAAGAATGGGC	50.0	50.0	58.0	58.1	2036-2051 bp	1.0	80.5
rpoA_p5	GTGGGCTTAGCGGTTTTAGA	GTAGTTGATCAGGGCATGCA	50.0	50.0	57.9	58.0	1857-1872 bp	1.0	80.2