Hotspot

rpl32-trnL-UAG

SSC · 110722-111449 · 728 bp

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Pi 0.012
MarkerSeek score 74.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
728
variable_sites
26
pi
0.012
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGGTTTCCACTGGACATTCA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.1 57.9 754-850 bp 1.0 84.7
rpl32-trnL-UAG_p2 CGGTTTCCACTGGACATTCA AGCAGCGTGTCTACCAATTT 50.0 45.0 58.1 57.8 747-843 bp 1.0 84.3
rpl32-trnL-UAG_p3 TCGGTTTCCACTGGACATTC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.8 57.9 755-851 bp 1.0 84.2
rpl32-trnL-UAG_p4 CGGTTTCCACTGGACATTCA CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.1 57.8 753-849 bp 1.0 84.2
rpl32-trnL-UAG_p5 CGGTTTCCACTGGACATTCA CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.1 57.8 752-848 bp 1.0 84.2