Hotspot

psaJ-rpl33

LSC · 65202-65721 · 520 bp

Back to Amana

Pi 0.0159
MarkerSeek score 72.50
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
520
variable_sites
27
pi
0.0159
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TTGGGTCTTTAGCGGGTCTA CGGGCACAACAACTAGTACA 50.0 50.0 58.1 57.8 621-674 bp 1.0 85.6
psaJ-rpl33_p2 ATGGTTTGGGTCTTTAGCGG CGGGCACAACAACTAGTACA 50.0 50.0 58.2 57.8 626-679 bp 1.0 84.7
psaJ-rpl33_p3 TGGGTCTTTAGCGGGTCTAT CGGGCACAACAACTAGTACA 50.0 50.0 57.8 57.8 620-673 bp 1.0 84.7
psaJ-rpl33_p4 TTGGGTCTTTAGCGGGTCTA TTTCGGGCACAACAACTAGT 50.0 45.0 58.1 57.7 624-677 bp 1.0 84.2
psaJ-rpl33_p5 ATGGTTTGGGTCTTTAGCGG TTTCGGGCACAACAACTAGT 50.0 45.0 58.2 57.7 629-682 bp 1.0 83.3