Hotspot

accD-psaI

LSC · 57222-58033 · 812 bp

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Pi 0.00804
MarkerSeek score 66.70
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
812
variable_sites
20
pi
0.00804
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 AAGAGGTGTTCTGAGTGAGT CCATTGCAATTGCCGGAAAT 45.0 45.0 55.7 58.0 698-968 bp 1.0 67.6
accD-psaI_p2 AGAGGTGTTCTGAGTGAGTT CCATTGCAATTGCCGGAAAT 45.0 45.0 55.7 58.0 697-967 bp 1.0 67.6
accD-psaI_p3 AAGAGGTGTTCTGAGTGAGT AAGAAGCCATTGCAATTGCC 45.0 45.0 55.7 57.9 704-974 bp 1.0 66.9
accD-psaI_p4 AGAGGTGTTCTGAGTGAGTT AAGAAGCCATTGCAATTGCC 45.0 45.0 55.7 57.9 703-973 bp 1.0 66.9
accD-psaI_p5 AAGAGGTGTTCTGAGTGAGTT CCATTGCAATTGCCGGAAAT 42.9 45.0 56.5 58.0 698-968 bp 1.0 65.9