Hotspot

psaA-ycf3

LSC · 40036-40637 · 602 bp

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Pi 0.00511
MarkerSeek score 61.90
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
602
variable_sites
11
pi
0.00511
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 CGAGAAATGACCTGGTCTGG TTGAAGATCACGAGGCGTTT 55.0 45.0 58.0 57.8 730-746 bp 1.0 81.2
psaA-ycf3_p2 CGAGAAATGACCTGGTCTGG TTGGTTGAAGATCACGAGGC 55.0 50.0 58.0 58.2 734-750 bp 1.0 80.9
psaA-ycf3_p3 GTCTGGCCCATTCCTCAAAA TTGAAGATCACGAGGCGTTT 50.0 45.0 58.1 57.8 716-732 bp 1.0 80.7
psaA-ycf3_p4 TGGCCCATTCCTCAAAAGAC TTGAAGATCACGAGGCGTTT 50.0 45.0 58.1 57.8 713-729 bp 0.9 76.0
psaA-ycf3_p5 TGGCCCATTCCTCAAAAGAC TTGGTTGAAGATCACGAGGC 50.0 50.0 58.1 58.2 717-733 bp 0.9 75.7