Hotspot

rpoB-trnC-GCA

LSC · 26131-26994 · 864 bp

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Pi 0.00612
MarkerSeek score 61.20
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
864
variable_sites
22
pi
0.00612
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AATTCTTCCGTCAAGCCCTG TGGGTCCACCATTTTGTCAA 50.0 45.0 58.2 57.5 1057-1065 bp 1.0 77.7
rpoB-trnC-GCA_p2 AATTCTTCCGTCAAGCCCTG GCGACACCCAGATTTGAACT 50.0 50.0 58.2 58.5 1033-1041 bp 1.0 77.7
rpoB-trnC-GCA_p3 AATTCTTCCGTCAAGCCCTG TAAAGGATTTGCAGTCCCCC 50.0 50.0 58.2 57.5 1008-1016 bp 1.0 77.3
rpoB-trnC-GCA_p4 AATTCTTCCGTCAAGCCCTG CCTTACCACTTGGCCATGTC 50.0 55.0 58.2 58.5 987-995 bp 1.0 77.3
rpoB-trnC-GCA_p5 AATTCTTCCGTCAAGCCCTG TTTGTCAATCAGGCGACACC 50.0 50.0 58.2 58.8 1045-1053 bp 1.0 75.3