Hotspot

trnS-GCU-trnR-UCU

LSC · 7828-9478 · 1651 bp

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Pi 0.00636
MarkerSeek score 63.40
Primer available yes
Label trnS-GCU-trnR-UCU

Feature Metadata

label_name
trnS-GCU-trnR-UCU
feature_type
igs
region
LSC
length_bp
1651
variable_sites
38
pi
0.00636
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnR-UCU_p1 TGATGAGGGATAGCGGAGAG AGAAGACCTCTGTCCTATCCA 55.0 47.6 58.1 56.9 1764-1821 bp 1.0 66.5
trnS-GCU-trnR-UCU_p2 TGATGAGGGATAGCGGAGAG AGGTTTAGAAGACCTCTGTCCT 55.0 45.5 58.1 57.9 1770-1827 bp 1.0 65.8
trnS-GCU-trnR-UCU_p3 GAGGGATAGCGGAGAGAGAG AGAAGACCTCTGTCCTATCCA 60.0 47.6 58.2 56.9 1760-1817 bp 1.0 65.7
trnS-GCU-trnR-UCU_p4 GATGAGGGATAGCGGAGAGA AGAAGACCTCTGTCCTATCCA 55.0 47.6 57.8 56.9 1763-1820 bp 1.0 65.5
trnS-GCU-trnR-UCU_p5 ATGAGGGATAGCGGAGAGAG AGAAGACCTCTGTCCTATCCA 55.0 47.6 57.8 56.9 1762-1819 bp 1.0 65.4