Hotspot

ndhF-rpl32

SSC · 109922-110631 · 710 bp

Back to Alyssum

Pi 0.0512
MarkerSeek score 78.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
710
variable_sites
53
pi
0.0512
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TCCATGAATATTGATATGTATGTTCCA ACGAATACGCTTTTTCGAGA 29.6 40.0 56.7 55.5 381-786 bp 1.0 50.3
ndhF-rpl32_p2 CCATGAATATTGATATGTATGTTCCA ACGAATACGCTTTTTCGAGA 30.8 40.0 55.5 55.5 380-785 bp 1.0 50.3
ndhF-rpl32_p3 TCCATGAATATTGATATGTATGTTCCA ACGAATACGCTTTTTCGAGAT 29.6 38.1 56.7 56.1 381-786 bp 1.0 50.3
ndhF-rpl32_p4 TCCATGAATATTGATATGTATGTTCC ACGAATACGCTTTTTCGAGA 30.8 40.0 55.3 55.5 381-786 bp 1.0 50.3
ndhF-rpl32_p5 CCATGAATATTGATATGTATGTTCCA ACGAATACGCTTTTTCGAGAT 30.8 38.1 55.5 56.1 380-785 bp 1.0 50.3