Hotspot

psaJ-rpl33

LSC · 64585-65011 · 427 bp

Back to Alyssum

Pi 0.0528

MarkerSeek score 67.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 427
variable_sites: 33
pi: 0.0528
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACCCGTGACATTTTGTACCC	GGGGTGTTATGCCGATTCTT	50.0	50.0	58.1	57.9	1036-1101 bp	1.0	86.3
psaJ-rpl33_p2	CCCGTGACATTTTGTACCCA	GGGGTGTTATGCCGATTCTT	50.0	50.0	58.1	57.9	1035-1100 bp	1.0	86.3
psaJ-rpl33_p3	ACCCGTGACATTTTGTACCC	TCCAATCGACTAGGGGTGTT	50.0	50.0	58.1	58.1	1048-1113 bp	1.0	86.2
psaJ-rpl33_p4	CCCGTGACATTTTGTACCCA	TCCAATCGACTAGGGGTGTT	50.0	50.0	58.1	58.1	1047-1112 bp	1.0	86.2
psaJ-rpl33_p5	ACCCGTGACATTTTGTACCC	TTCCAATCGACTAGGGGTGT	50.0	50.0	58.1	58.1	1103-1114 bp	0.5	60.4