Hotspot

trnW-UGG-trnP-CCA

LSC · 63842-64030 · 189 bp

Back to Alyssum

Pi 0.0441

MarkerSeek score 69.40

Primer available yes

Label trnW-UGG-trnP-CCA

Feature Metadata

label_name: trnW-UGG-trnP-CCA
feature_type: igs
region: LSC
length_bp: 189
variable_sites: 13
pi: 0.0441
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-UGG-trnP-CCA_p1	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.0	58.1	250-258 bp	1.0	87.0
trnW-UGG-trnP-CCA_p2	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	50.0	50.0	58.0	58.1	248-256 bp	1.0	87.0
trnW-UGG-trnP-CCA_p3	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	50.0	50.0	58.0	58.1	251-259 bp	1.0	86.9
trnW-UGG-trnP-CCA_p4	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	261-269 bp	1.0	86.8
trnW-UGG-trnP-CCA_p5	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	50.0	50.0	58.0	58.1	262-270 bp	1.0	86.8