Hotspot

petA-psbJ

LSC · 60646-61608 · 963 bp

Back to Alyssum

Pi 0.0355

MarkerSeek score 70.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 963
variable_sites: 50
pi: 0.0355
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	CTGGACGGATTCCTCTTTGG	50.0	55.0	58.0	58.0	1098-1203 bp	1.0	86.9
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	CTGGACGGATTCCTCTTTGG	50.0	55.0	58.2	58.0	1095-1200 bp	1.0	85.8
petA-psbJ_p3	TTACGTGTCCAAGGCCTTTT	CTGGACGGATTCCTCTTTGG	45.0	55.0	57.6	58.0	1093-1198 bp	1.0	84.4
petA-psbJ_p4	TACGTGTCCAAGGCCTTTTG	CTGGACGGATTCCTCTTTGG	50.0	55.0	58.4	58.0	1092-1197 bp	1.0	84.1
petA-psbJ_p5	TGTCCAAGGCCTTTTGTTCT	CTGGACGGATTCCTCTTTGG	45.0	55.0	57.5	58.0	1088-1193 bp	1.0	83.7