Hotspot

ndhC-trnV-GUA

LSC · 48687-49422 · 736 bp

Back to Alyssum

Pi 0.0349

MarkerSeek score 74.60

Primer available yes

Label ndhC-trnV-GUA

Feature Metadata

label_name: ndhC-trnV-GUA
feature_type: igs
region: LSC
length_bp: 736
variable_sites: 41
pi: 0.0349
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-GUA_p1	GCCCAGAAAATATCATATTCGT	GGTTCGAATCCGTATAGCCC	36.4	55.0	54.4	57.9	793-872 bp	1.0	48.0
ndhC-trnV-GUA_p2	GCCCAGAAAATATCATATTCGT	CGGTTCGAATCCGTATAGCC	36.4	55.0	54.4	58.3	794-873 bp	1.0	48.0
ndhC-trnV-GUA_p3	GCCCAGAAAATATCATATTCGT	CAGGTCTACGGTTCGAATCC	36.4	55.0	54.4	57.8	802-881 bp	1.0	47.9
ndhC-trnV-GUA_p4	GCCCAGAAAATATCATATTCGT	GCAGGTCTACGGTTCGAATC	36.4	55.0	54.4	58.4	803-882 bp	1.0	47.9
ndhC-trnV-GUA_p5	GCCCAGAAAATATCATATTCGT	AGCAGGTCTACGGTTCGAAT	36.4	50.0	54.4	58.8	804-883 bp	1.0	47.9