Hotspot

psbM-trnD-GAC

LSC · 27838-28876 · 1039 bp

Back to Alyssum

Pi 0.045

MarkerSeek score 72.10

Primer available yes

Label psbM-trnD-GAC

Feature Metadata

label_name: psbM-trnD-GAC
feature_type: igs
region: LSC
length_bp: 1039
variable_sites: 71
pi: 0.045
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GAC_p1	AGGAACGAGAATAAAGAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.5	58.5	1133-1157 bp	1.0	64.9
psbM-trnD-GAC_p2	GGAACGAGAATAAAGAGTGCAG	TTCAATTGGTCAGAGCACCG	45.5	50.0	57.2	58.5	1132-1156 bp	1.0	62.4
psbM-trnD-GAC_p3	GGAACGAGAATAAAGAGTGCA	TTCAATTGGTCAGAGCACCG	42.9	50.0	56.0	58.5	1132-1156 bp	1.0	61.2
psbM-trnD-GAC_p4	CGAGAATAAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.5	50.0	57.0	58.5	1128-1152 bp	1.0	61.0
psbM-trnD-GAC_p5	AGGAACGAGAATAAAGAGTGCA	GTTCAATTGGTCAGAGCACC	40.9	50.0	57.5	57.0	1134-1158 bp	1.0	60.7