Hotspot

trnR-AGA-atpA

LSC · 9141-9377 · 237 bp

Back to Alyssum

Pi 0.0633

MarkerSeek score 67.20

Primer available yes

Label trnR-AGA-atpA

Feature Metadata

label_name: trnR-AGA-atpA
feature_type: igs
region: LSC
length_bp: 237
variable_sites: 25
pi: 0.0633
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-AGA-atpA_p1	AGCCTTCCAAGCTAACGATG	GGGTCGTGAAGCTTATCCAG	50.0	55.0	58.0	58.1	1208-1304 bp	1.0	86.0
trnR-AGA-atpA_p2	AGCCTTCCAAGCTAACGATG	GGTCGTGAAGCTTATCCAGG	50.0	55.0	58.0	58.1	1207-1303 bp	1.0	86.0
trnR-AGA-atpA_p3	CGAAAGGCGTCCATTGTCTA	GGGTCGTGAAGCTTATCCAG	50.0	55.0	58.0	58.1	994-1114 bp	1.0	84.7
trnR-AGA-atpA_p4	CGAAAGGCGTCCATTGTCTA	GGTCGTGAAGCTTATCCAGG	50.0	55.0	58.0	58.1	993-1113 bp	1.0	84.7
trnR-AGA-atpA_p5	TTCTGTCAAAGCACCCAGAC	GGTCGTGAAGCTTATCCAGG	50.0	55.0	58.0	58.1	1400-1446 bp	0.5	60.8