Hotspot

trnS-AGC-trnG-GGA

LSC · 7373-8110 · 738 bp

Back to Alyssum

Pi 0.0576
MarkerSeek score 80.70
Primer available yes
Label trnS-AGC-trnG-GGA

Feature Metadata

label_name
trnS-AGC-trnG-GGA
feature_type
igs
region
LSC
length_bp
738
variable_sites
61
pi
0.0576
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.85
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-AGC-trnG-GGA_p1 CCAACGCTTTAGTCCACTCA GGAACGAATCACACTTTTACCA 50.0 40.9 57.8 56.9 661-807 bp 1.0 64.5
trnS-AGC-trnG-GGA_p2 AACGCTTTAGTCCACTCAGC GGAACGAATCACACTTTTACCA 50.0 40.9 58.2 56.9 659-805 bp 1.0 64.2
trnS-AGC-trnG-GGA_p3 CCAACGCTTTAGTCCACTCA GGAACGAATCACACTTTTACCAC 50.0 43.5 57.8 58.2 661-807 bp 1.0 63.4
trnS-AGC-trnG-GGA_p4 AACGCTTTAGTCCACTCAGC GGAACGAATCACACTTTTACCAC 50.0 43.5 58.2 58.2 659-805 bp 1.0 63.1
trnS-AGC-trnG-GGA_p5 CCAACGCTTTAGTCCACTCA AGGAACGAATCACACTTTTACCA 50.0 39.1 57.8 58.3 662-808 bp 1.0 62.9