Hotspot

trnK-AAA-rps16

LSC · 4403-5123 · 721 bp

Back to Alyssum

Pi 0.0634
MarkerSeek score 80.70
Primer available yes
Label trnK-AAA-rps16

Feature Metadata

label_name
trnK-AAA-rps16
feature_type
igs
region
LSC
length_bp
721
variable_sites
68
pi
0.0634
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.92
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-AAA-rps16_p1 CCTTTCAGGATCAGTCGTGG GCCTTGTTGCTGTCGTTTTT 55.0 45.0 58.0 58.1 1358-1424 bp 1.0 90.0
trnK-AAA-rps16_p2 TCGACACCGAAAACAAGGTT GCCTTGTTGCTGTCGTTTTT 45.0 45.0 58.0 58.1 1450-1516 bp 1.0 89.8
trnK-AAA-rps16_p3 TGGTCTTACAAACGCTACCG GCCTTGTTGCTGTCGTTTTT 50.0 45.0 57.9 58.1 1341-1407 bp 1.0 89.6
trnK-AAA-rps16_p4 TCAGGATCAGTCGTGGTCTT GCCTTGTTGCTGTCGTTTTT 50.0 45.0 58.1 58.1 1354-1420 bp 1.0 89.4
trnK-AAA-rps16_p5 TTCAGGATCAGTCGTGGTCT GCCTTGTTGCTGTCGTTTTT 50.0 45.0 58.1 58.1 1355-1421 bp 1.0 89.4