Hotspot

ycf1

SSC · 123152-128614 · 5463 bp

Back to Alternanthera

Pi 0.0196

MarkerSeek score 53.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5463
variable_sites: 107
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTCACGGAAGAAGTTGTCCA	AACGGCGTTCAATTATCCGA	50.0	45.0	57.8	58.0	506 bp	0.5	58.0
ycf1_p2	GTCACGGAAGAAGTTGTCCA	ACGGCGTTCAATTATCCGAA	50.0	45.0	57.8	58.0	505 bp	0.5	58.0
ycf1_p3	TGTCACGGAAGAAGTTGTCC	AACGGCGTTCAATTATCCGA	50.0	45.0	57.8	58.0	507 bp	0.5	58.0
ycf1_p4	TGTCACGGAAGAAGTTGTCC	ACGGCGTTCAATTATCCGAA	50.0	45.0	57.8	58.0	506 bp	0.5	58.0
ycf1_p5	GTCACGGAAGAAGTTGTCCA	GGTCTGAAGATTTCGCCGAT	50.0	50.0	57.8	58.1	559 bp	0.5	57.7