Hotspot

rpl16

LSC · 81516-82972 · 1457 bp

Back to Alternanthera

Pi 0.0172

MarkerSeek score 55.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1457
variable_sites: 25
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GCACCAAAAATGCGAGTACC	CAAAAACATCCGCTGGTGTC	50.0	50.0	58.0	57.9	2552-2564 bp	1.0	80.9
rpl16_p2	ACACATCAATTCTCGAGCCC	CAAAAACATCCGCTGGTGTC	50.0	50.0	58.0	57.9	2313-2325 bp	1.0	80.9
rpl16_p3	TCGACGATTGCTAGCTCCTA	CAAAAACATCCGCTGGTGTC	50.0	50.0	58.0	57.9	2343-2355 bp	1.0	80.9
rpl16_p4	ACACATCAATTCTCGAGCCC	CTCTACAAACCATTCGGGCA	50.0	50.0	58.0	57.9	1911-1923 bp	1.0	80.6
rpl16_p5	GCACCAAAAATGCGAGTACC	CTCTACAAACCATTCGGGCA	50.0	50.0	58.0	57.9	2150-2162 bp	1.0	80.5